Isosmotic Striction of Rat Aorta Smooth Muscle Cells During Activation of Purinergic Receptors: Role of Chlorine Transport

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We studied the effect of the purinergic signaling system and Cl-transporters on vascular smooth muscle cells (SMC) isosmotic striction that occurs when osmotic pressure is normalized after prolonged incubation in a hypoosmotic medium. The study was performed with the method of myography on endothelium-denuded ring segments of the male Wistar rats aorta. Isosmotic striction was induced by placing the vascular segments in normosmotic Krebs solution containing 120 mM NaCl after a 40-minute incubation in a hyposmotic Krebs solution containing 40 mM NaCl. Purinergic receptors were activated by adenosine 5'-triphosphate (ATP, 500 μM) as nonselective P2X and P2Y receptor agonist, and uridine 5'-triphosphate (UTP, 500 μM) as selective P2Y receptor agonist. ATP and UTP eliminated the transient nature of the aorta SMC isosmotic striction without affecting its amplitude. Pretreatment of vascular segments with ATP and UTP during incubation in a hyposmotic solution completely suppressed the development of isosmotic striction in the presence of ATP or UTP, but did not affect isosmotic striction without activators of purinergic receptors. The inhibitor of Na+, K+, 2Cl--cotransport (NKCC) bumetanide (100 μM) abolished isosmotic striction in the presence of ATP, but not UTP, but restored its transient character. A non-selective blocker of Cl– channels and Cl–, HCO3– exchanger DIDS (100 μM) suppressed the development of isosmotic striction both in the presence of ATP and UTP. The potassium channel blocker tetraethylammonium (10 mM) potentiates the constrictor action of UTP on isosmotic striction. We suppose purinergic receptors eliminate the transient isosmotic striction by activating Cl– currents through activation of P2Y receptors. The mechanism of interaction between the purinergic signaling system and Cl– transport during changes in cell volume requires further study.

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L. Smaglii

Siberian State Medical University; Tomsk State University; Seversk biophysical scientific center

编辑信件的主要联系方式.
Email: lud.smagly@yandex.ru
俄罗斯联邦, Tomsk; Tomsk; Seversk

V. Gusakova

Siberian State Medical University

Email: lud.smagly@yandex.ru
俄罗斯联邦, Tomsk

S. Gusakova

Siberian State Medical University

Email: lud.smagly@yandex.ru
俄罗斯联邦, Tomsk

M. Pshemyskiy

Siberian State Medical University

Email: lud.smagly@yandex.ru
俄罗斯联邦, Tomsk

S. Koshuba

Siberian State Medical University

Email: lud.smagly@yandex.ru
俄罗斯联邦, Tomsk

E. Golovanov

Siberian State Medical University

Email: lud.smagly@yandex.ru
俄罗斯联邦, Tomsk

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2. Fig. 1. The effect of purinergic receptor activators on the development of isoosmotic striction of the smooth muscle segments of the rat aorta in a series of experiments described in the section "Research methods" (detailed description of the results in the text). (a) – the effect of ATP (500 microns) and UTF (500 microns) on the isoosmotic friction obtained according to the first experimental scheme. (b) – the effect of ATP (500 µm) and UTF (500 µm) on the isoosmotic friction obtained according to the second experimental scheme. (c) – the effect of ATP (500 microns) and UTF (500 microns) on the isoosmotic friction obtained according to the third experimental scheme. On the abscissa axis is time (hours), on the ordinate axis is mechanical stress (mN). The arrows show the addition and removal of solutions. The dotted line shows the change in the mechanical stress of the segment in the control, the solid line shows the change when ATP or UTF is added. The diagrams show the median and interquartile range of the amplitude of the isoosmotic friction in the corresponding series of experiments. * – statistically significant about

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3. Fig. 2. The effect of the NKCC inhibitor bumetanide (100 µm, pretreatment 15 min) and the inhibitor of Cl-channels and Cl-/HCO3-exchanger DIDS (100 µm, pretreatment 30 min) on the development of isoosmotic striction of the smooth muscle segments of the rat aorta in the presence of 500 µm ATP (a) or 500 µm UTF (b). Bumetanide and DIDS were added 15 or 30 minutes, respectively, before induction of isoosmotic striction. On the abscissa axis – time (h), on the ordinate axis – mechanical stress (mN). The arrows show the addition and removal of solutions.

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4. Fig. 3. The effect of TEA (10 mM, pretreatment 10 min) on the amplitude and duration of isoosmotic aortic stricture in the presence of ATP (a) or UTF (b). A solid line shows the control isoosmotic striction in the absence of additives, dots – isoosmotic striction in the presence of TEA, dotted lines – isoosmotic striction in the presence of ATP or UTF, dotted line with a dot – the same, but with the action of TEA. TEA was added 10 minutes before the induction of isoosmotic striction. On the ordinate axis – mechanical stress (mN), on the abscissa axis - time (h).

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5. Fig. 4. A hypothetical scheme of interaction between the purinergic signaling system and the mechanisms of regulatory increase in cell volume during isoosmotic striction (explanations in the text). NKCC – Na+, K+, 2Cl--cotransport, CC – chlorine channels (without specifying the type), CaSS – calcium-activated chlorine channels, CaPC – calcium-activated potassium channels, CaC – potential-dependent calcium channels, NCa – Na+, Ca2+ exchanger, ATP - adenosine-5’-triphosphate, UTP – uridine-5’-triphosphate, P2X receptors, P2Y receptors, SPR – sarcoplasmic reticulum, IP3 – inositol-triphosphate, DAG – diacylglycerol, PKC – protein kinase C, ECl, hypo – equilibrium potential Cl – during incubation in a hypoosmotic medium; ECl, iso – The equilibrium potential of Cl is when returning to the isoosmotic medium. The blue arrows show the activating effect, the red ones show the inhibitory effect.

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