Laboratory System for Intensive Cultivation of Microalgae and Cyanobacteria

D. A. Gabrielyan; Габриелян Д. А.; M. A. Sinetova; Синетова М. А.; A. K. Gabrielyan; Габриелян А. К.; L. A. Bobrovnikova; Бобровникова Л. А.; V. S. Bedbenov; Бедбенов В. С.; A. Y. Starikov; Стариков А. Ю.; A. A. Zorina; Зорина А. А.; B. V. Gabel; Габель Б. В.; D. A. Los; Лось Д. А.

doi:10.31857/S0015330322600486

Laboratory System for Intensive Cultivation of Microalgae and Cyanobacteria

Авторлар: Gabrielyan D.A.¹, Sinetova M.A.¹, Gabrielyan A.K.¹, Bobrovnikova L.A.¹, Bedbenov V.S.¹, Starikov A.Y.¹, Zorina A.A.¹, Gabel B.V.¹, Los D.A.¹
Мекемелер:
1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences
Шығарылым: Том 70, № 2 (2023)
Беттер: 202-213
Бөлім: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
URL: https://modernonco.orscience.ru/0015-3303/article/view/648160
DOI: https://doi.org/10.31857/S0015330322600486
EDN: https://elibrary.ru/GKCNLD
ID: 648160

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат
Рұқсат жабық

Рұқсат берілді
Рұқсат жабық

Тек жазылушылар үшін

Аннотация
Авторлар туралы
Әдебиет тізімі
Қосымша файлдар
Статистика

Аннотация

Currently, microalgae and cyanobacteria attract the attention of researchers as potential producers of various valuable substances. To increase the profitability of biotechnological processes using these organisms, it is necessary to select highly effective strains and choose the optimal conditions for their growth and maximum productivity. Growth optimization should be carried out, on the one hand, under intensive conditions, as close as possible to large-scale cultivation, and, on the other hand, in small volumes in order to be able to check many different parameters in parallel at minimal cost. In this paper, the authors present a description and characteristics of their laboratory system for intensive cultivation (LSIC—Laboratory System for Intensive Cultivation) with thermo-, light-, and gas regulation and the possibility of cultivation in four repetitions in eight different conditions, differing in light, temperature, and CO₂ concentration. As an example, the results of a number of experiments using the installation are also presented.

Негізгі сөздер

biomass, biotechnology, microalgae, growth optimization, LED lighting, strain screening, cyanobacteria

Әдебиет тізімі

Spolaore P., Joannis-Cassan C., Duran E., Isambert A. Commercial applications of microalgae // J. Biosci. Bioeng. 2006. V. 101. P. 87. https://doi.org/10.1263/jbb.101.87
Chisti Y. Raceways-based production of algal crude oil // Green. 2013. V. 3. P. 195. https://doi.org/10.1515/green-2013-0018
Hoang A.T., Sirohi R., Pandey A., Nižetić S., Lam S.S., Chen W.-H., Luque R., Thomas S., Arıcı M., Pham V.V. Biofuel production from microalgae: challenges and chances // Phytochem. Rev. 2022. https://doi.org/10.1007/s11101-022-09819-y
Zorina A.A., Bedbenov V.S., Novikova G.V., Panichkin V.B., Los D.A. Involvement of serine/threonine protein kinases in the cold stress response in the cyanobacterium Synechocystis sp. PCC 6803: Functional characterization of SpkE protein kinase // Mol. Biol. 2014. V. 48(3). P. 390. https://doi.org/10.1134/S0026893314030212
Sinetova M.A., Los D.A. Systemic analysis of stress transcriptomics of Synechocystis reveals common stress genes and their universal triggers // Mol. BioSyst. 2016. V. 12. P. 3254. https://doi.org/10.1039/C6MB00551A
Mironov K.S., Sinetova M.A., Shumskaya M., Los D.A. Universal molecular triggers of stress responses in cyanobacterium Synechocystis // Life. 2019. V. 9. P. 67. https://doi.org/10.3390/life9030067
Tsygankov A.A. Laboratory scale photobioreactors. // Appl. Biochem. Microbiol. 2001. V. 37(4). P. 333. https://doi.org/10.1023/A:1010266116747
Benner P., Meier L., Pfeffer A., Kruger K., Oropeza Vargas J.E., Weuster-Botz D. Lab-scale photobioreactor systems: principles, applications, and scalability // Bioprocess Biosyst. Eng. 2022. V. 45. P. 791. https://doi.org/10.1007/s00449-022-02711-1
Владимирова М.Г., Семененко В.Е. Интенсивная культура одноклеточных водорослей. М.: Академия наук СССР, 1962. 58 с.
Абдуллаев А.А., Семененко В.Е. Интенсивная культура Dunaliella salina Teod. и некоторые ее физиологические характеристики // Физиология растений. 1974. Т. 21. P. 1145.
Fuchs T., Arnold N.D., Garbe D., Deimel S., Lorenzen J., Masri M., Mehlmer N., Weuster-Botz D., Bruck T.B. A newly designed automatically controlled, sterilizable flat panel photobioreactor for axenic algae culture // Front. Bioeng. Biotechnol. 2021. V. 9. P. 697354. https://doi.org/10.3389/fbioe.2021.697354
Sinetova M.A., Sidorov R.A., Starikov A.Y., Voronkov A.S., Medvedeva A.S., Krivova Z.V., Pakholkova M.S., Bachin D.V., Bedbenov V.S., Gabrielyan D.A., Zayadan B.K., Bolatkhan K., Los D.A. Assessment of biotechnological potential of cyanobacteria and microalgae strains from the IPPAS culture collection // Appl. Biochem. Microbiol. 2020. V. 56. P. 36. https://doi.org/10.1134/S0003683820070030
Nowicka-Krawczyk P., Mühlsteinová R., Hauer T. Detailed characterization of the Arthrospira type species separating commercially grown taxa into the new genus Limnospira (Cyanobacteria) // Sci. Rep. 2019. V. 9. P. 694. https://doi.org/10.1038/s41598-018-36831-0
Furmaniak M.A., Misztak A.E., Franczuk M.D., Wilmotte A., Waleron M., Waleron K.F. Edible cyanobacterial genus Arthrospira: Actual state of the art in cultivation methods, genetics, and application in medicine // Front. Microbiol. 2017. V. 8. P. 2541. https://doi.org/10.3389/fmicb.2017.02541
Fučíková K., Lewis L. Intersection of Chlorella, Muriella and Bracteacoccus: Resurrecting the genus Chromochloris Kol et Chodat (Chlorophyceae, Chlorophyta) // Fottea. 2012. V. 12. P. 83. https://doi.org/10.5507/fot.2012.007
Liu J., Sun Z., Gerken H., Liu Z., Jiang Y., Chen F. Chlorella zofingiensis as an alternative microalgal producer of astaxanthin: biology and industrial potential // Mar. Drugs. 2014. V. 12. P. 3487. https://doi.org/10.3390/md12063487
Mahajan G., Kamat M. γ-Linolenic acid production from Spirulina platensis // Appl. Microbiol. Biotechnol. 1995. V. 43. P. 466. https://doi.org/10.1007/bf00218450
Golmakani M.T., Rezaei K., Mazidi S., Razavi S.H. γ-Linolenic acid production by Arthrospira platensis using different carbon sources // Eur. J. Lipid Sci. Technol. 2012. V. 114. P. 306. https://doi.org/10.1002/ejlt.201100264