Transcriptional activity of genes regulating neurogliogenesis and apoptosis in rats trained in morris water maze: the influence of stress and spatial memory formation

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Дәйексөз келтіру

Толық мәтін

Аннотация

The formation of new neural networks and the modification of pre-existing synaptic contacts, which underlie learning and memory, largely depend on the activity of genes involved in the regulation of the associated processes of neurogliogenesis and apoptosis. At the same time, the identification of changes in the functioning of the genome specific to cognitive functions requires a simultaneous assessment of the influence of stressful factors as a persistent component of all models of laboratory animal learning. The aim of this study was to compare the expression of genes regulating neurogliogenesis (S100А6, Ascl1), and apoptosis (Apaf1, Bax, Casp3, Bcl2) in animals trained in spatial Morris maze and those subjected to forced swimming in accordance with the training regime. The experiments were conducted on young adult male Wistar rats, distributed into the following groups: Training (trained to find a hidden platform in a water maze for 4 days), Control (swimming in a maze without a platform for 4 days) and Intact (staying in home cages). In tissue samples of the hippocampus, prefrontal cortex, and cerebellum obtained immediately after the end of experiments in the maze, the expression of target genes was determined by real-time polymerase chain reaction. It was found that cognitive activity reduces the expression of pro-apoptotic genes, which increases under stress conditions, and, on the contrary, stimulates the activity of genes regulating neurogliogenesis and synaptogenesis in structures relevant for various stages of memory trace formation. The results obtained, are of interest for understanding molecular mechanisms of stress and cognition as well as for determining the targets of therapy for cognitive disorders.

Негізгі сөздер

Авторлар туралы

A. Ratmirov

Federal Research Center for Innovator and Perspective Biomedical and Pharmaceutical Technologies

Moscow, Russia

M. Gruden

Federal Research Center for Innovator and Perspective Biomedical and Pharmaceutical Technologies

Moscow, Russia

Z. Storozheva

Federal Research Center for Innovator and Perspective Biomedical and Pharmaceutical Technologies

Email: storozheva_zi@academpharm.ru
Moscow, Russia

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