Detection of Malassezia Fungi in Microplankton and Fishes in the Severnaya Bay, Sevastopol (Russia)

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In this study, the microplankton and mycobiota of fish in Severnaya Bay, Sevastopol, was assessed using real-time PCR (RT-PCR). Microplankton samples were collected using a Biber-2 sequential filtration setup, and microbiota samples were obtained from the surface of fishes (Trachurus mediterraneus and Spicara smaris). DNA was extracted by alkaline lysis during high-temperature incubation and analyzed for the presence of 13 species of fungal pathogens from the genera Candida, Malassezia, Saccharomyces, and Debaryomyces. A positive result was obtained for Malassezia spp. in the planktonic fraction (150–300 µm), and a signal was detected from the surface of Trachurus mediterraneus. Other fungal pathogens, including Candida albicans, Debaryomyces hansenii, and Meyerozyma guilliermondii, were not detected. The results emphasize the role of microplankton and fish as potential reservoirs of fungal pathogens in marine ecosystems.

作者简介

N. Kolbasov

Sevastopol State University

Email: kiku200@yandex.ru
Sevastopol, Russia

Yu. Podkidysheva

Pirogov Russian National Research Medical University

Moscow, Russia

A. Shevyrev

“DNA-technology” Company

Moscow, Russia

A. Kuznetsov

Sevastopol State University; A. O. Kovalevsky Institute of Biology of the Southern Seas of the Russian Academy of Sciences

Email: kuznet61@gmail.com
Sevastopol, Russia; Sevastopol, Russia

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