COMPLEX STUDY OF THE INTERNAL STRUCTURE OF POLYMERIC SPONGE MATRIXES IN THE PROCESS OF A TISSUE-ENGINEERED ARRAGEMENT

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Abstract

The article presents the approaches of a complex study of the 3D structure of a polymeric spongy matrix based on polylactide using X-ray microtomography without staining in combination with a non-invasive method of scanning pulsed acoustic microscopy and energy-dispersive X-ray microanalysis. Comparative studies of pure matrix samples, in vitro samples kept in a culture medium and after their implantation in vivo were conducted. The use of a complex of non-invasive methods revealed changes in the microstructure, including matrix degradation processes in a culture medium, after incubation with cells and implantation in a laboratory animal.

About the authors

Y. S. Krivonosov

National Research Center “Kurchatov Institute”

Moscow, Russia

Y. S. Petronyuk

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Moscow, Russia

E. A. Khramtsova

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Moscow, Russia

T. D. Patsaev

National Research Center “Kurchatov Institute”

Moscow, Russia

A. M. Azieva

National Research Center “Kurchatov Institute”

Moscow, Russia

M. Y. Kopaeva

National Research Center “Kurchatov Institute”

Moscow, Russia

D. A. Kirillova

National Research Center “Kurchatov Institute”

Moscow, Russia

K. G. Antipova

National Research Center “Kurchatov Institute”

Moscow, Russia

N. A. Sharikova

National Research Center “Kurchatov Institute”

Moscow, Russia

T. E. Grigoriev

National Research Center “Kurchatov Institute”; Moscow Institute of Physics and Technology; Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences

Moscow, Russia; Moscow, Russia; Moscow, Russia

V. E. Asadchikov

National Research Center “Kurchatov Institute”

Moscow, Russia

A. L. Vasiliev

National Research Center “Kurchatov Institute”; Moscow Institute of Physics and Technology

Email: a.vasiliev56@gmail.com
Moscow, Russia; Moscow, Russia

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