Comparative assessment of the combined action of chlorsulfuron-based pesticides

Introduction. For several decades, herbicides have remained effective means of weed control, accounting for more than 60% of registered pesticide preparations. Among modern herbicides, combination preparations are popular. The range of combination preparations continues to increase, and currently there are more than fifty different names. Given the specific features of the joint interaction of pesticides, the requirements for their toxicological and hygienic assessment are increasing from year to year.Materials and methods. To establish the nature of the combined action of chlorsulfuron with other active substances and study the nature and degree of interaction of these substances, the method of orthogonal experimental design using probability values was used.Results. Based on the results of the studies, toxicity parameters were established and the nature and degree of interaction of various combinations of chlorsulfuron with other active substances: 2,4-D and dicamba were determined.Limitations. When choosing toxicometric parameters for chlorsulfuronic acid, the intragroup number of rats is 6–10 individuals, depending on the tasks being solved, which is a sufficient reference sample.Conclusion. The parameters of acute oral toxicity were established and the interdependent additive nature of the combined action of chlorsulfuron with other active substances was determined upon a single oral administration to the body of laboratory animals.Compliance with ethical standards. Study approved of the Ethics Committee of the Federal Research Center of Hygiene named after F.F. Erisman (Protocol of the Ethics Commission № 01/22 dated 27.20.2022). The study was conducted in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (ETS N 123), Directive of the European Parliament and of the Council of the European Union 2010/63/EC of 22.09.2010 on the protection of animals used for scientific purposes.Contribution: Bagreev A.Yu. – design of the study, collection and processing of material, writing the text; Rakitskiy V.N. — research concept and design, editing. All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.Conflict of interest. The authors declare no conflict of interest.Funding. The study had no sponsorship.Received: February 2, 2025 / Revised: March 3, 2025 / Accepted: March 26, 2025 / Published: July 31, 2025

toxicological studies, chlorsulfuron, sulfonylurea derivatives